FastQCFastQC Report
Sun 19 Mar 2023
SRR4895683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578233
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG199633.4524145111053848No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45430.785669444670228No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27760.480083288224643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG22810.39447765866009027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAATAGAAATTTATGTAG10950.18937002903673777No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTTAGTTTGTGGCTTCAT8300.14354075260318938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA8290.1433678119374024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7600.13143490599810112No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGAAGAAATGCAAACTAT6930.11984788139037378No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6770.11708083073778217No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA6610.11431378008519057No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTCTATGTTCATTCTATA6110.10566674679584181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT342900.0102.580281
AGCAGTG354600.099.072982
AGTGGTA353450.099.046655
GGTATCA355350.098.863888
GTGGTAT354150.098.748526
GTATCAA356200.098.6076059
CAGTGGT355350.098.517064
TGGTATC356850.098.143587
GCAGTGG360950.096.928353
GTTACGC259.3870866E-486.9945145
AGGGGTA1807.2759576E-1140.275235
GAGGTAT2404.3655746E-1133.2270666
AGGTATC2558.913048E-1131.2725327
GGGGTAT2401.5788828E-930.2064256
AGAGGTA2651.382432E-1030.0924365
GATATCA5750.027.7373777
AGTGATA5800.027.4982624
GTGATAT6000.026.5816545
GGGTATC2808.187271E-925.8912187
TGATATC6200.025.7241786
Produced by FastQC (version 0.11.5)