FastQCFastQC Report
Sun 19 Mar 2023
SRR4895682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979826
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG659196.7276230677691755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA102361.0446752790801632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG37050.37812836156623725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33040.337202727831268No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC26130.26668000236776734No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA16500.16839724604164413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT15150.1546192895473278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC13080.13349308958937606No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA11210.11440806837132309No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTGAGTTAGATCGGAAGAGCAC10520.10736600171867251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT491500.060.8316351
AGTGGTA505300.058.992055
AGCAGTG509700.058.5711482
CAGTGGT512200.058.2316064
GCAGTGG520200.057.2803233
GTGGTAT521550.057.1481976
GGTATCA525050.057.032528
TGGTATC525650.056.7743077
GTATCAA528650.056.6961069
CATGGCG5000.036.249233145
CGGTCTG3200.031.71808145
AAATATA21500.025.627367145
CGACCTA3300.023.72628845-49
TACGCAC3600.023.35966550-54
GCCCCAA10750.022.929749145
AAACCGC9200.022.69447785-89
CACGGGA3554.125468E-922.464315145
CCGCAAG11000.022.408617145
CCCGGGT2704.445519E-621.481028145
GAAACCG9600.021.44680885-89
Produced by FastQC (version 0.11.5)