FastQCFastQC Report
Sun 19 Mar 2023
SRR4895681_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895681_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003460
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACGCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT61220.6100890917425706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA1004300.0144.117142
CACGCAT356300.0143.955863
TTCACGC1005700.0143.916521
ACGCAGT117200.0143.685414
ACGCATA104100.0143.61574
ACGCATG127750.0143.56184
ACGCATT121950.0143.115594
CACGCAG332650.0143.068483
CGCATTG42350.0142.706575
CACGCAA299100.0142.647833
CGCATAG34300.0142.557135
ACGCAAT108550.0142.511814
CGCATTA36400.0141.861665
CGCAGTA35650.0141.811655
ACGCAGG114750.0141.473864
CGCATAT35350.0141.3835
CGCATGA29750.0141.087785
ACGCAAG105050.0141.08124
CGCAGTT39950.0140.989785
CGCAATG36350.0140.866365
Produced by FastQC (version 0.11.5)