FastQCFastQC Report
Sun 19 Mar 2023
SRR4895681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003460
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG308493.0742630498475276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA47800.47635182269348053No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG41970.41825284515576105No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33880.337631793992785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC22120.2204372869870249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT293400.077.101681
GGTATCA306350.074.4740458
AGCAGTG305200.074.140752
GTGGTAT304700.074.0207756
AGTGGTA305300.073.922795
GTATCAA308900.073.882729
CAGTGGT308100.073.325234
TGGTATC309550.073.071817
GCAGTGG311900.072.3389053
TTCACGC3050.042.7899741
TCACGCA2900.042.500952
AGGTATC4150.034.9387977
AAATATA24600.027.997198145
CGGTCTG3900.027.88385145
GAGGTAT4950.027.827526
CGACCTA3150.025.77706745-49
AGAGGTA5500.025.0447645
TACGCAC3150.023.93584850-54
CATGGGG59000.023.1010625-29
GAAACCG9750.022.45579385-89
Produced by FastQC (version 0.11.5)