FastQCFastQC Report
Sun 19 Mar 2023
SRR4895680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006075
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG175411.7435081877593617No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28280.2810923638893721No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA24970.24819223218944908No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC23640.2349725418085133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG23250.23109609124568248No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT20560.20435852197897772No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACGCGCTTCAACTTCGGT10740.10675148473026366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT250300.093.3941
AGTGGTA255050.091.186185
GTGGTAT255600.090.928726
GGTATCA256800.090.786118
CAGTGGT257750.090.296214
GTATCAA258700.090.0913249
AGCAGTG259200.090.042832
TGGTATC260000.089.445697
GCAGTGG265900.087.4740453
CGGTCTG2109.094947E-1237.97273145
CCGCAAG11950.035.185078145
CGGAGCC5150.026.745134145
CATGGGG63300.023.1601125-29
CGCTGTT4455.456968E-1222.806908145
CGACCTA2550.021.60694745-49
ATGGGGG24200.020.9722925-29
TACGCAC2700.020.94274350-54
TCCCGTA1051.8189894E-1220.71260550-54
CGGCGGT3100.020.11266935-39
TACATGG244050.019.68364520-24
Produced by FastQC (version 0.11.5)