FastQCFastQC Report
Sun 19 Mar 2023
SRR4895679_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895679_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991750
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT136211.3734308041341063No Hit
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT24500.24703806402823292No Hit
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT15100.1522561129316864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA994050.0143.887182
GCTTCAG335650.0143.683783
TGGCTTC997500.0143.389511
GCTTCAT374900.0143.162983
GCTTCAA271150.0142.891163
CTTCATG154150.0142.209354
CTTCAGT121750.0141.674064
CTTCAGA95200.0141.64554
CTTCAGG120200.0141.401254
CTTCATA105100.0139.898364
CTTCAAT103350.0138.569264
TTCAGTA34150.0137.674975
CTTCAAG94050.0137.550374
TCATGTA50800.0136.981436
TTCATAG37600.0136.357775
TTCAGAT33650.0136.077645
TCAGGTA11550.0135.480216
TTCAGGA34250.0135.378145
TTCATGA31050.0135.163535
CTTCATT121950.0134.759894
Produced by FastQC (version 0.11.5)