FastQCFastQC Report
Sun 19 Mar 2023
SRR4895679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991750
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG373503.766070078144694No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA71230.7182253592135115No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT67250.6780942777917822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC32150.3241744391227628No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27390.27617847239727755No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26820.27043105621376357No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC18210.18361482228384168No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT14120.1423745903705571No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA13210.13319889084950845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC11830.11928409377363247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC10660.10748676581799849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT381750.079.215671
AGCAGTG393200.076.742962
AGTGGTA401850.074.856485
GTGGTAT403500.074.510686
GGTATCA405550.074.366438
GTATCAA407050.074.088659
TGGTATC406500.074.014297
CAGTGGT408300.073.74874
GCAGTGG416000.072.29653
CCGCAAG17300.032.685463145
CGGTCTG3150.032.219856145
AGAGAGT34650.030.755318145
TACGCAC4450.025.41284250-54
CGACCTA4850.024.51242845-49
GCCCCAA14550.022.919281145
AAATATA20050.022.417307145
CGATAGG653.9990482E-822.3071835-39
CATGGGG78400.022.15526225-29
TCTCGCA5350.021.9534370-74
CCTATCG604.556823E-721.75279105-109
Produced by FastQC (version 0.11.5)