FastQCFastQC Report
Sun 19 Mar 2023
SRR4895678_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895678_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714449
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAACA716650.0143.759161
GAACAAT263000.0143.307173
GGAACAA720950.0142.901722
GAACAAG251000.0141.655173
AACAATG94700.0140.100284
AACAAGT90050.0139.007174
ACAATGG34950.0136.372075
GAACAAA207450.0135.557113
ACAAGTG31500.0135.513445
ACAATGT33500.0135.171775
CAATGTG12750.0135.163336
ACAATTG34800.0134.266285
ACAAGTA28400.0134.056175
CAATTAG10450.0133.861796
ACAATGA27200.0133.3435
ACAATAG29250.0133.11875
CAATAGG11650.0132.452166
AACAAGG98150.0132.384054
ACAAGTT29300.0130.18455
CAATGAG10600.0129.246546
Produced by FastQC (version 0.11.5)