FastQCFastQC Report
Sun 19 Mar 2023
SRR4895678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714449
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG207102.8987373486421006No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA28640.4008683614925628No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28300.39610944937987175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC14610.20449325284240022No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC8720.1220520988901937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8080.11309414667806938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT211750.076.605551
AGTGGTA213550.075.67235
GTGGTAT214150.075.426446
GGTATCA215250.075.2430658
GTATCAA216000.075.015379
AGCAGTG216000.074.89162
CAGTGGT217450.074.358864
TGGTATC217400.074.298857
GCAGTGG220700.073.2313
GGTCTGA5450.033.255997145
GAGGTAT2502.4447218E-928.999236
CGGTCTG3050.028.523834145
CACGCGA1050.007708001427.618315145
CGACCTA2900.024.99933645-49
CGCAAGG4950.024.898329145
CCGCGAA2800.024.33966390-94
TACGCAC2950.024.08410650-54
CATGGGG43850.023.47714225-29
CGCGAAA2800.023.30393290-94
CCGCAAG8850.022.937244145
Produced by FastQC (version 0.11.5)