FastQCFastQC Report
Sun 19 Mar 2023
SRR4895677_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895677_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442543
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCTCG100.007206879144.205989
TGAAGAG445050.0143.379731
GAAGAGA451600.0141.300162
AAGAGAT155450.0139.567643
AAGAGAG165900.0136.774033
AAGAGAA141100.0128.058183
AGAGATG62150.0126.919834
AGAGAGT58700.0126.518034
GAGAGTC5300.0125.1599045
AGAGATT56650.0123.841514
GAGATTG21200.0123.799475
GAGATTA16350.0123.0381245
AGAGATA53500.0122.5077064
GAGATGA16850.0121.954625
GAGATGT23550.0121.243255
GAGAGTT18950.0120.615575
AGAGAGG72000.0120.371944
AGAGAAT50900.0119.8411254
GAGATAG18250.0118.5254755
GAGAGTG21050.0118.173555
Produced by FastQC (version 0.11.5)