FastQCFastQC Report
Sun 19 Mar 2023
SRR4895677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442543
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG75561.7074047041756395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20310.458938453438423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10910.24652971575643495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG8890.20088443382902904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8850.19998056686016952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5130.11592093875623385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT93750.086.156851
GGTATCA95200.085.215778
AGTGGTA94950.085.144355
GTGGTAT94900.085.103196
CAGTGGT96400.083.713224
GTATCAA96800.083.582569
AGCAGTG96700.083.528492
TGGTATC97350.083.035877
GCAGTGG97750.082.2603763
CGGTCTG1351.9099389E-1048.33209145
GCCCCAA4050.034.01147145
AGAGAGT7250.024.999357145
GTCGGTA300.001495068824.16604490-94
TACGCAC1550.023.38649450-54
CATGGGG20900.023.2410325-29
TCTCGCA1650.022.8504870-74
TCGCGGT403.105224E-421.74944135-39
CGACCTA1550.021.51557445-49
GTTAGCG350.00366065920.71375385-89
CGCGAAA801.6347258E-819.93698790-94
Produced by FastQC (version 0.11.5)