FastQCFastQC Report
Sun 19 Mar 2023
SRR4895676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474548
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG374897.8999384677630085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA64411.3572915700835322No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24320.5124876724799178No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT18210.3837335738428989No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA9800.20651230223286157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7800.16436693443023678No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA6650.1401333479437275No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG5230.11021013680386389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT315050.066.323171
AGTGGTA318650.065.339385
AGCAGTG323050.064.642662
GTGGTAT323100.064.432686
GGTATCA324100.064.368128
CAGTGGT324200.064.2567444
GTATCAA325000.064.198649
TGGTATC324500.064.154697
GCAGTGG330950.062.946183
CATGCGT750.001482928438.664925145
CGTGGGA4750.035.103683145
CGGGCTC1051.8527427E-434.52225145
CATGCGA5100.034.116108145
CAGGCGA1102.4348736E-432.95306145
CCGGCGT1804.694777E-628.193174145
CCGCAAG5550.027.431198145
CGCAAGG3800.026.709324145
CGTGCGC2101.5533742E-524.165577145
CGACCTA1450.023.99891945-49
GGGTAGT851.8189894E-1223.88278825-29
Produced by FastQC (version 0.11.5)