FastQCFastQC Report
Sun 19 Mar 2023
SRR4895675_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895675_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891942
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT9910.1111058790818237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG310350.0144.162813
CCGTCTA892700.0144.080032
CGTCTAT315050.0143.82023
TCCGTCT895050.0143.701751
CGTCTAA252500.0143.695253
GTCTATG117250.0143.040854
GTCTAAG94200.0142.908024
GTCTAGA87100.0142.719164
GTCTAGT109100.0142.68924
GTCTAGG113950.0142.374224
GTCTAAT88600.0142.011964
GTCTATA90450.0141.578584
TCTAGAG29900.0141.55665
GTCTATT108800.0140.034094
GTCTAAA71700.0138.979934
TCTATAG34000.0138.907465
CTAGATG10700.0138.818276
CGTCTAC16500.0138.52833
TCTAGAT30600.0138.318375
CTAGAGT9750.0137.553456
Produced by FastQC (version 0.11.5)