FastQCFastQC Report
Sun 19 Mar 2023
SRR4895675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891942
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG179862.016498830641454No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36330.4073134800244859No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT29250.32793612140699735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA25270.2833143859129854No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG19080.21391525457933364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC18760.21032757735368443No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC11940.13386520648203584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGTGGGGTTTTCTTCTA11380.12758677133714974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT228700.090.313261
AGTGGTA232500.088.124945
GTGGTAT233550.087.69776
GGTATCA235100.087.3970648
AGCAGTG235650.087.316142
CAGTGGT234950.087.298564
GTATCAA236700.087.046459
TGGTATC237750.086.209457
GCAGTGG243650.084.092133
CGGTCTG3150.041.427353145
TACGCAC2850.026.4556650-54
CCGCAAG11300.025.021389145
CGACCTA2950.023.5933245-49
CATGGGG60150.023.52715525-29
CGCTGTT5500.022.408432145
AGAGAGT17650.021.769897145
CGATAGG1300.021.19287535-39
CGCGAAA2500.020.87938790-94
CGGCGGT3450.020.59475135-39
GGTCTGA6350.020.550577145
Produced by FastQC (version 0.11.5)