FastQCFastQC Report
Sun 19 Mar 2023
SRR4895674_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895674_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321316
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTGGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT61841.9245851435969574No Hit
ACATTGGCGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATACCACT3450.10737093702149908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGC321800.0144.003572
ACATTGG322000.0143.914121
ATTGGCG129300.0143.614183
ATTGGCT100550.0143.367033
ATTGGCA83900.0143.110293
TTGGCGA32850.0142.471484
TTGGCGT55900.0140.744524
TTGGCTA28950.0140.74034
TTGGCAT29050.0140.504064
GGCATAG3400.0139.985696
TGGCGTA11700.0139.913165
TTGGCTG39850.0139.522634
TGGCGAT10300.0139.326725
TGGCTAG10150.0139.25435
TGGCTGA10100.0139.229685
TGGCATG11200.0139.076685
TTGGCAG31950.0139.036384
TGGCGAA9700.0139.023595
TGGCATA8100.0138.885915
TGGCTAA9600.0137.4675
Produced by FastQC (version 0.11.5)