FastQCFastQC Report
Sun 19 Mar 2023
SRR4895674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321316
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG184825.751970023279264No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23880.7431936162531589No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11350.3532348217953665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10380.32304647138642334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9570.2978376426944192No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG7910.24617510488117617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6990.2175428550087764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCA6180.19233402631677227No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5530.17210471934170724No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4850.1509417520447161No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT3840.11950852120653811No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3390.10550361637764692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA3320.10332507562648607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT168800.066.341941
GTGGTAT170600.065.058896
GGTATCA172250.064.772398
GTATCAA172400.064.7160349
AGTGGTA171550.064.666425
TGGTATC171900.064.566887
AGCAGTG173650.064.395462
CAGTGGT173450.064.1770554
GCAGTGG174250.063.7991753
CGTGGGA2309.986252E-1031.519741145
AAATATA6200.029.23202145
GCGCTAG200.00607280129.00267670-74
GGTCGCG255.157125E-428.99816550-54
CGCTGTT1855.802902E-627.430695145
TAGGTCG351.2567293E-424.8555745-49
ACGTACC300.001493863424.16889870-74
AGAGGTA1801.5162045E-424.1688985
GCCCCAA3001.699118E-824.165136145
AAACCGC2450.023.6719785-89
AGGTATC1851.827689E-423.5120247
Produced by FastQC (version 0.11.5)