FastQCFastQC Report
Sun 19 Mar 2023
SRR4895673_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895673_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932093
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAGCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT62780.6735379409565354No Hit
TATCAGCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT10690.11468812661397522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC932550.0144.143541
ATCAGCA933000.0144.074022
TCAGCAT329300.0144.001883
TCAGCAG325200.0143.888243
CAGCATG128300.0143.321584
TCAGCAA262600.0143.177373
CAGCAGT122150.0143.040164
CAGCATT107950.0142.350464
CAGCATA93400.0142.29074
CAGCAAT93300.0142.056784
AGCATGT56050.0141.390495
CAGCAAG93450.0141.134264
CAGCAGG116600.0140.633884
AGCAGTA36300.0139.850525
AGCAGTG40250.0139.741965
AGCAGAT30050.0139.181535
AGCATGG40950.0139.114145
AGCATGA28700.0138.944495
AGCATTG39350.0138.90655
AGCAGTT41050.0138.775245
Produced by FastQC (version 0.11.5)