FastQCFastQC Report
Sun 19 Mar 2023
SRR4895673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932093
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG363053.8949976021705988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA56750.6088448255699807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36890.3957759579784421No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC12730.13657435470494897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT10790.11576098093216021No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGTGGGGTTTTCTTCTA10750.11533183920488621No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA10670.11447355575033823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT315000.071.989921
AGTGGTA319700.070.485615
AGCAGTG326100.069.346842
GGTATCA327000.069.244678
GTATCAA327900.069.1577459
CAGTGGT326750.068.9874
GTGGTAT326400.068.9721156
TGGTATC329550.068.356857
GCAGTGG334250.067.417353
CGGTCTG3500.043.50008145
ACGCGCA900.003627120332.224013
GGTCTGA5950.030.462242145
CGGAGCC5250.026.238146145
AATAGTT9100.024.697847145
CGCAAGG6500.022.307734145
CATGGGG59200.022.14122225-29
CGCGAAA3650.021.45117290-94
CCGCAAG10200.021.323568145
TGCGGCT8550.021.198872145
ATAGGCG3650.021.05664100-104
Produced by FastQC (version 0.11.5)