FastQCFastQC Report
Sun 19 Mar 2023
SRR4895672_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895672_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380354
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG381200.0143.888241
AGGATGA381800.0143.662142
GGATGAT145300.0142.671433
GGATGAG114350.0142.50733
GGATGAA118900.0142.147933
GATGATG50200.0138.177124
GGATGAC6700.0137.752663
GATGAAG39750.0137.316764
GATGAGT45450.0136.118824
GATGATA44800.0135.679534
ATGATGG16550.0134.624855
GATGATT56650.0134.409154
GATGAGA34950.0134.306994
GATGAAT48000.0134.29544
ATGAGTA13250.0132.781865
GATGAGG39850.0132.086784
ATGATGT20200.0131.359425
ATGATTG20950.0130.786945
ATGAAGT14400.0130.690145
ATGATGA13850.0130.153265
Produced by FastQC (version 0.11.5)