FastQCFastQC Report
Sun 19 Mar 2023
SRR4895672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380354
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG88782.334141352529486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17270.4540507001372406No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13110.3446789043890691No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT5390.1417100911256356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGTGGGGTTTTCTTCTA5320.1398697003317962No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5110.1343485279502779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT101000.086.352941
AGTGGTA102800.084.547715
GGTATCA103500.083.905858
GTGGTAT103400.083.846796
GTATCAA104300.083.609789
AGCAGTG104850.082.9055562
CAGTGGT105400.082.404154
TGGTATC106400.081.618957
GCAGTGG106850.081.218043
CGGTCTG1109.9487806E-846.129757145
GCCCCAA4000.034.432568145
AATAGTT4550.027.084034145
TTAGCGG1250.024.36163585-89
TACGCAC2200.023.72699450-54
CATGGGG21200.023.4596325-29
CGGCGGT2250.023.19789735-39
CGACCTA2250.023.19728545-49
GTTAGCG1200.022.95987385-89
TGCGGCT2953.8666622E-722.115475145
AGAGAGT4701.0913936E-1121.592651145
Produced by FastQC (version 0.11.5)