FastQCFastQC Report
Sun 19 Mar 2023
SRR4895671_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895671_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456656
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT9340.20453032479590766No Hit
GTGTTCTAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT5320.11649907151115939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG152900.0143.42783
TGTTCTA459400.0143.366242
GTGTTCT462200.0142.497741
GTTCTAA130550.0142.461963
GTTCTAT168450.0142.046263
TTCTAGA40150.0141.53544
TCTAGAG13300.0140.97635
TTCTAGG58500.0139.66854
CTAGAGT4400.0139.31276
TTCTAAG47650.0138.93264
TCTAGAT14900.0138.42175
CTAGTTG6900.0137.958766
TTCTATG65750.0137.758474
TCTAAGG16450.0137.65385
TCTAAGT16950.0137.422325
TCTATAG16850.0137.381915
TCTAGGA14200.0137.11975
TCTAGGT21150.0137.069275
TTCTAGT56850.0136.872254
TCTAGTG18400.0136.782975
Produced by FastQC (version 0.11.5)