FastQCFastQC Report
Sun 19 Mar 2023
SRR4895671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456656
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG242805.316912511825094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA36720.8041063732875513No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT15920.3486212816649732No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15150.3317595739462528No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11510.2520496829123016No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA7270.15920079885077607No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC5350.1171560211625381No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4770.10445499456921623No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACGCGCTTCAACTTCGGT4660.10204617918082759No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4640.1016082127465751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT217950.072.201761
AGTGGTA223350.070.383475
AGCAGTG224850.070.018352
GGTATCA227200.069.3032768
CAGTGGT227250.069.183154
GTGGTAT226950.069.156026
GTATCAA227900.069.090419
TGGTATC229050.068.553627
GCAGTGG233300.067.4823153
CCGCAAG6000.036.247017145
CGGTCTG1707.8125595E-834.114838145
CGGAGCC2506.91216E-1131.897375145
CACGTCC1409.991053E-425.890726145
CGCAAGG3709.094947E-1225.470877145
TACGCAC1300.023.42114850-54
CGACCTA1250.023.19859945-49
CATGGGG32750.022.67031125-29
AGAGAGT11950.022.445852145
ATGGGGC12100.020.8527225-29
GGTCTGA2455.109441E-520.71258145
Produced by FastQC (version 0.11.5)