FastQCFastQC Report
Sun 19 Mar 2023
SRR4895670_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895670_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608552
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11890.1953818243962718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCA609450.0144.008212
GTCTGTC610400.0143.784071
CTGTCAT214050.0143.682163
CTGTCAA182500.0143.6683
CTGTCAG204350.0143.621283
TGTCAGA58950.0142.386324
GTCAATG21800.0142.236485
TGTCAAG67050.0142.177784
TGTCAGT72600.0141.936694
TGTCAGG72450.0141.931964
TGTCATG80300.0141.706744
TGTCATA62850.0141.582294
TGTCAAT62250.0141.556854
GTCATGA20450.0140.694995
GTCATAG22300.0140.340795
GTCAGAT20250.0140.304065
GTCAAGT23500.0139.925225
GTCAGTA21350.0139.830375
GTCAGTT23750.0139.666845
GTCAAGA19550.0139.057255
Produced by FastQC (version 0.11.5)