FastQCFastQC Report
Sun 19 Mar 2023
SRR4895670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608552
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG101001.659677398151678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19880.3266770957946075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15350.2522380996200818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11910.1957104733860048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9280.15249313123611458No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7770.12768013251127266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7530.12373634463447658No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC7180.11798498731414901No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT6680.10976876257082387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT141900.090.76121
AGTGGTA143850.089.213735
GTGGTAT144200.089.032846
GGTATCA144950.089.022288
GTATCAA145850.088.522659
AGCAGTG145950.087.979752
TGGTATC146400.087.7939457
CAGTGGT146950.087.381044
GCAGTGG151300.084.725013
CGCTGTT4000.036.249546145
CGGTCTG2252.8136128E-828.99964145
CGATAGG300.001495368124.16557135-39
CGACCTA2200.023.72541645-49
CATGGGG39950.023.59092125-29
CTATCGC2150.022.256268140-144
TACGCAC2350.022.21248850-54
TATATCG2350.022.21212490-94
AGAGAGT9850.021.34491145
ATATCGC2700.020.9438490-94
CGTGGGA2806.0844704E-620.71403145
Produced by FastQC (version 0.11.5)