FastQCFastQC Report
Sun 19 Mar 2023
SRR4895669_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895669_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583827
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC43440.744056030296646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG584150.0144.13841
TCGTAGA584350.0144.089072
CGTAGAG191700.0143.848653
CGTAGAT224200.0143.645863
CGTAGAA160350.0143.59523
GTAGATG80250.0142.517494
GTAGAGT67850.0141.567764
GTAGATA74050.0139.550424
GTAGAAG59700.0139.393174
GTAGAGG68550.0139.385774
GTAGAGA58100.0139.135994
GTAGAAT56950.0137.893624
GTAGATT73500.0137.55324
CGTAGAC10150.0137.120153
TAGATGA22700.0135.965265
TAGAGTG22650.0134.991885
TAGATGG29850.0134.80315
TAGATAG20050.0134.513925
TAGATGC3600.0134.209215
TAGAGGT23000.0132.937655
Produced by FastQC (version 0.11.5)