FastQCFastQC Report
Sun 19 Mar 2023
SRR4895668_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895668_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716587
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGCAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT132741.8523919635717645No Hit
GTACGCAATGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC20690.2887297704256427No Hit
GTACGCAAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC19790.276170234737722No Hit
GTACGCAAGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA16290.2273275959513639No Hit
GTACGCAAGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATACCACT7180.10019718471030035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAA717250.0144.092222
GTACGCA717400.0144.06211
CGCAATG96150.0143.782964
ACGCAAT249400.0143.770323
ACGCAAG265350.0143.472023
CGCAAGT111400.0143.456154
CGCAATA70150.0142.485344
CGCAATT81750.0142.11584
CGCAAAT67900.0141.896384
ACGCAAA195700.0141.800843
GCAAGTG33300.0141.63425
CGCAAGG91000.0141.221544
GCAATTG30200.0141.128655
GCAAGAT24350.0140.6795
GCAATGT35300.0140.555665
CGCAAAG74200.0140.053744
GCAAGTT51150.0139.862295
GCAATGG35450.0139.757495
GCAAGGT30050.0139.193245
CGCAAGA64350.0138.965744
Produced by FastQC (version 0.11.5)