FastQCFastQC Report
Sun 19 Mar 2023
SRR4895668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716587
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG513637.167727017096319No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA70780.9877377066566934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21040.29361403430427846No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA13090.18267146906097934No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTTGC11680.16299486314990363No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11460.1599247544261897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11270.15727329689207312No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC9880.13787579177406234No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8910.12433940331041451No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC7750.10815155731265011No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG7300.10187178946868979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT423400.052.6925931
GTATCAA425650.052.4918989
AGTGGTA426000.052.146095
GTGGTAT426100.052.11326
GGTATCA428350.052.076418
TGGTATC429900.051.7200167
CAGTGGT434100.051.1897854
AGCAGTG436700.050.9846272
GCAGTGG442100.050.2470863
CGTGGGA8450.036.03337145
CCGCAAG7700.032.01109145
CGGAGCC3351.8189894E-1228.132664145
CGGTCTG2401.5978494E-624.165237145
CGACCTA2200.023.72719445-49
CGTGCGG1851.8306954E-423.512123145
CGCGAAA1450.021.99992890-94
CTTGGGA12750.021.606562145
TACGCAC2500.021.4590350-54
CATGGGG45600.020.86045325-29
CCGCGAA1700.020.47051890-94
Produced by FastQC (version 0.11.5)