FastQCFastQC Report
Sun 19 Mar 2023
SRR4895667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776716
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG173572.234664922571442No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23520.3028133835275699No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22480.2894236760926774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC17760.22865500388816506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT12400.15964651172371883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT214850.082.9887851
GGTATCA217300.081.9249048
AGTGGTA217550.081.66415
GTGGTAT217400.081.648486
GTATCAA218000.081.513059
TGGTATC219150.081.034797
CAGTGGT221250.080.429524
AGCAGTG221550.080.418812
GCAGTGG229350.077.462513
CGGTCTG3050.035.653633145
CCGCAAG8600.028.661098145
TACGCAC2700.023.09122850-54
CATGGGG47250.023.01747725-29
CGACCTA2900.021.99870145-49
ATGGGGA17750.020.42395825-29
CGGAGCC3651.360022E-719.86184145
GGTCTGA4102.1662345E-819.450071145
CGCGAAA2250.019.33219190-94
TGCGGCT5305.638867E-1119.149813145
ATGGGAG22400.018.9678425-29
Produced by FastQC (version 0.11.5)