FastQCFastQC Report
Sun 19 Mar 2023
SRR4895666_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895666_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172779
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAC1178550.0143.495671
GAGAACA1179950.0143.32542
AGAACAT412000.0142.892783
GAACAGT151600.0142.398014
AGAACAG416150.0142.247483
GAACATG142550.0142.232684
GAACATT150550.0141.09234
GAACATA120350.0140.311114
GAACAAT125950.0139.969064
AACAGTG52350.0138.971515
GAACAGG151700.0138.311654
GAACAGA116200.0138.310534
AGAACAA351450.0138.173693
GAACAAG129050.0137.053734
AACATGG53750.0134.010355
AACATTG56850.0133.80535
ACAGTAG15900.0133.775386
ACAGTGT18300.0133.567236
AACATGA41600.0133.459265
AACATGT50600.0133.09075
Produced by FastQC (version 0.11.5)