FastQCFastQC Report
Sun 19 Mar 2023
SRR4895666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172779
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG137201.169870879338733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40320.3437987890301583No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG20450.17437215366236947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA18410.15697757207453408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT18090.1542490102568344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC13260.11306478032093004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC12250.10445275708381545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT224300.099.194731
GTGGTAT223550.099.17086
GGTATCA225450.098.9460148
GTATCAA226450.098.248769
AGTGGTA225900.098.1433265
TGGTATC228300.097.107457
AGCAGTG229400.096.9303742
CAGTGGT229250.096.772434
GCAGTGG235000.094.250333
GGTCTGA6750.032.220963145
CGGTCTG3850.030.128695145
GAGGTAT3555.456968E-1226.5482586
CGGAGCC5600.024.597252145
CGACCTA4500.024.48793245-49
CATGGGG70500.023.71507825-29
TACGCAC4900.023.67274750-54
CGCTGTT6200.023.386183145
CGATAGG1050.022.09625835-39
TGCGGCT9200.022.064354145
CGCGAAA3000.021.7491590-94
Produced by FastQC (version 0.11.5)