FastQCFastQC Report
Sun 19 Mar 2023
SRR4895665_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895665_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700041
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGGTAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT16080.22970083180842266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA700650.0144.048952
GCTCGGT701350.0143.905181
TCGGTAT243400.0143.886543
TCGGTAG237450.0143.666583
TCGGTAA208150.0143.524663
CGGTATA70150.0143.463334
CGGTAGT85650.0143.425194
CGGTATG88750.0143.04554
CGGTAAG72750.0142.59724
CGGTAAT73900.0142.524324
CGGTATT84000.0142.122964
CGGTAGA62600.0141.764314
CGGTAGG88950.0141.751314
CGGTAAA62900.0139.712814
TCGGTAC13750.0139.4643
GGTATAG26000.0134.755395
GGTAATG29100.0134.5215
GGTAGTA28200.0133.701355
GGTATGA25750.0131.584235
GGTATTG33550.0130.215685
Produced by FastQC (version 0.11.5)