FastQCFastQC Report
Sun 19 Mar 2023
SRR4895665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700041
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG157212.245725607500132No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG46860.6693893643372316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC21790.31126748290457273No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21430.30612492696856325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21240.3034108002245583No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC17330.24755692880845548No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11260.16084772177629597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT257050.099.6075061
GGTATCA262800.096.883458
GTATCAA263500.096.797979
GTGGTAT262750.096.68116
CAGTGGT263900.096.3422244
TGGTATC264550.096.0780947
AGCAGTG265650.096.0760042
AGTGGTA267800.094.939185
GCAGTGG272100.093.46553
CCGCAAG6150.056.578983145
GCTATCC2200.042.83608145
AAATATA19400.039.608932145
CGCCGAA1157.145167E-637.82182145
CGGTCTG850.002742943434.113796145
CGCAAGG3200.031.715172145
AGGGGTA1658.401046E-526.370085
TGCGATA1450.025.99929630-34
CCGCGCT2559.455289E-825.5944923
CGTAAGG1700.025.589369100-104
GCTATTC1701.0316506E-425.585348145
Produced by FastQC (version 0.11.5)