FastQCFastQC Report
Sun 19 Mar 2023
SRR4895664_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895664_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880666
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA881050.0144.144552
GCTAACG881200.0144.120011
TAACGAT304950.0143.83173
TAACGAG310400.0143.536353
TAACGAA252950.0143.326323
AACGAGT102450.0142.872794
AACGATG113200.0142.42654
ACGATAT31550.0142.381685
ACGATTG35700.0141.98835
AACGAAT89700.0141.959244
AACGATT100300.0141.550114
AACGATA93400.0141.430824
ACGAATG32200.0141.298955
ACGAGTT33300.0141.178575
ACGATAG32700.0141.122965
ACGATGT38200.0140.623645
ACGATGA32050.0140.61045
ACGAGTA29600.0140.312455
ACGAGTG34850.0140.071995
ACGATGG40450.0139.931855
Produced by FastQC (version 0.11.5)