FastQCFastQC Report
Sun 19 Mar 2023
SRR4895664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880666
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG193362.1956110489107106No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28350.3219154594363811No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27370.31078751762870377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA246850.086.013465
AAGCAGT248500.085.807081
GGTATCA250300.085.28668
GTGGTAT250700.084.62516
GTATCAA253600.084.3054059
TGGTATC253900.083.591837
AGCAGTG255100.083.37392
CAGTGGT254950.083.252294
GCAGTGG260800.081.384863
CGGAGCC4350.031.664274145
GGTCTGA6500.023.421307145
CGGTCTG2850.022.893007145
CATGGGG49850.022.63126425-29
CTAACGA1952.6107163E-422.312342
GCTAACG2104.3124644E-420.7150731
CGACCTA2400.019.93599345-49
CGCGAAA2450.019.52913790-94
ATGGGAG28200.019.23166325-29
ATGGGGG19500.019.03705225-29
CGGTATC2307.952801E-418.915917
Produced by FastQC (version 0.11.5)