FastQCFastQC Report
Sun 19 Mar 2023
SRR4895663_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895663_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699410
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACTGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT124061.773780758067514No Hit
ACCACTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC57840.826982742597332No Hit
ACCACTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA8450.12081611644100027No Hit
ACCACTGTGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATACCACT8040.11495403268469138No Hit
ACCACTGTGTTGATACCACTGCTGCCCATGTACTCTGCGTTGATACCACT8020.11466807737950557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGTA201100.0143.780763
CCACTGT701750.0143.72812
CACTGTG263400.0143.581423
ACTGTGA74250.0143.434144
ACTGTAG74650.0143.341724
ACTGTGT100700.0142.922164
CACTGTT220700.0142.806173
ACTGTTG94900.0141.855644
ACTGTGG85850.0141.691334
ACTGTAA65250.0141.448364
ACTGTTA64250.0141.293154
CTGTATG22700.0141.034585
CTGTAGT24500.0140.973655
ACTGTAT58450.0140.633534
CTGTAGA21400.0140.504675
CTGTTGA39050.0138.856225
CTGTAAG25850.0138.632265
CTGTGAG26550.0138.507785
CTGTTAG27550.0137.929615
CTGTGAA22450.0137.46625
Produced by FastQC (version 0.11.5)