FastQCFastQC Report
Sun 19 Mar 2023
SRR4895663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699410
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG590408.4414006090848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA73141.0457385510644686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26540.3794626899815559No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG26150.3738865615304328No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT18860.26965585279020887No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA14970.2140375459315709No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAG14130.2020274231137673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC13680.19559342874708682No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA10980.1569894625470039No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10000.1429776525928997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA8070.11538296564247007No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG7990.11423914442172689No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7190.10280093221429491No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGTGCGGTGGCTCAT7130.1019430662987375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT492700.063.0606041
GTATCAA501150.062.0897189
GGTATCA503050.061.826398
GTGGTAT502600.061.62216
TGGTATC503800.061.4753277
AGCAGTG507750.061.1442342
AGTGGTA512400.060.504455
GCAGTGG513200.060.396013
CAGTGGT519100.059.7374954
CGGTCTG1255.1932147E-946.3989145
CTTGCGA1351.0255462E-842.961945145
CGCTGTT2600.041.825935145
GCTATCC2950.034.405968145
CGTGGGG4250.034.116837145
CGTGGGA9600.032.47319145
CATGCGA1801.291719E-732.22146145
TGCGGCT2901.2732926E-1129.99929145
CGTGCGG1753.7731752E-628.999315145
CCTGCGG3700.027.431784145
AGGTATC3204.1836756E-1127.1868577
Produced by FastQC (version 0.11.5)