FastQCFastQC Report
Sun 19 Mar 2023
SRR4895662_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895662_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076638
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA1077350.0144.116871
CGAGTAA1077650.0144.076742
GAGTAAG392250.0143.316483
GAGTAAT378150.0143.092213
GAGTAAA301700.0141.086263
AGTAAGT139000.0139.496484
AGTAAGG149550.0139.491974
AGTAATG144450.0139.375084
AGTAAGA111900.0138.610964
AGTAATA113650.0137.428314
AGTAAAG120950.0136.645694
GTAAGTA40100.0135.765625
GTAAGTG49100.0135.405615
GTAATGG55500.0134.2135
AGTAATT131950.0134.052644
AGTAAAT110800.0133.869544
GTAATAG43300.0133.72575
GTAATGA40050.0133.414475
GTAAGGT47700.0132.577075
GTAATTG48900.0131.977955
Produced by FastQC (version 0.11.5)