FastQCFastQC Report
Sun 19 Mar 2023
SRR4895662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076638
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG169241.5719303981468238No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36390.3379966153897596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23570.21892223755802784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC19570.1817695455668479No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT15660.14545278914546952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT227800.093.515991
GGTATCA229900.092.518438
AGTGGTA229300.092.412745
GTGGTAT230350.091.928566
GTATCAA232450.091.3787469
TGGTATC233400.090.789397
AGCAGTG235700.090.157882
CAGTGGT235950.089.939454
GCAGTGG243550.087.073333
CGGTCTG3000.045.915535145
CCGCAAG11400.026.07392145
CGCAAGG6950.025.035353145
GCTATCC3902.0008883E-1124.166069145
CATGGGG70350.023.5396325-29
TACGCAC2700.023.0913850-54
CGATAGG2200.023.0680435-39
ATACGGT650.00798995822.3061057
CGACCTA2650.021.88584745-49
GAGGTAT3004.558715E-721.7484536
TCGTAAG2450.020.713198100-104
Produced by FastQC (version 0.11.5)