FastQCFastQC Report
Sun 19 Mar 2023
SRR4895661_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895661_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631961
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACAT632900.0144.01471
CCACATA634650.0143.617582
CACATAG225950.0143.592853
CACATAT214450.0142.617033
ACATAGT77150.0142.174764
ACATAGG83000.0142.145924
ACATATG77500.0141.811834
CACATAA185050.0141.503223
CATAGGT27250.0141.32015
CATAGTG24700.0141.019565
CATAGAG21500.0140.876975
ACATAGA66150.0140.74264
CATATGG27250.0140.261525
CATAGTA26150.0139.26725
ACATAAG66350.0139.122794
CATAATG22800.0138.854145
CATAGGA21900.0138.633185
CATAGAT21750.0138.594575
CATATAG24100.0137.648035
CATATGT27800.0137.227165
Produced by FastQC (version 0.11.5)