FastQCFastQC Report
Sun 19 Mar 2023
SRR4895661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631961
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG199713.1601633645114173No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG68701.0870923996892214No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC41240.652571915039061No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA28560.4519266220542091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20590.32581124468123823No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8400.13291959472182618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT221650.085.092951
GGTATCA225300.083.8620458
CAGTGGT225450.083.375824
GTATCAA227050.083.1771559
GTGGTAT226400.083.006256
AGCAGTG227850.082.720392
TGGTATC228650.082.2211467
AGTGGTA229950.081.7441945
GCAGTGG232300.080.948463
AAATATA22700.051.737686145
TATTCGA657.333342E-444.6168488
TTATTCG657.333342E-444.6168487
AGGGGTA1601.8717201E-631.727325
GGGGTAT1606.822705E-527.188396
AAACCGC13950.025.36083285-89
GATAAGT14700.024.7581495-99
CGCAGAT15300.024.3550890-94
GAAACCG14700.024.26418185-89
CATGGGG42350.023.79841225-29
CCAGCGC15300.023.31264345-49
Produced by FastQC (version 0.11.5)