FastQCFastQC Report
Sun 19 Mar 2023
SRR4895660_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895660_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483196
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT110102.278578465053519No Hit
GATAGACATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT11490.23779170357370508No Hit
GATAGACATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT9990.20674840023510127No Hit
GATAGACATGTACTCTGCGTTGATACCACTGCGTCCCATGTACTCTGCGT9180.18998501643225524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC483550.0144.092651
ATAGACA484250.0143.884352
TAGACAT187200.0143.865263
TAGACAG158650.0143.484743
AGACATG87650.0143.389284
GACATGT49650.0143.340585
TAGACAA132900.0142.421513
AGACATT54900.0142.110494
AGACAGT56450.0141.912734
GACAATG17650.0141.760765
ACATGTA34550.0141.707536
GACATGC2550.0141.384265
GACATGG20300.0141.370335
AGACAAT46650.0141.275154
GACATTA16000.0140.606645
GACAGAT14200.0140.149635
GACATTG19700.0139.81975
GACAAGT15850.0139.207735
GACAGTA16650.0139.015125
AGACATA45650.0138.999474
Produced by FastQC (version 0.11.5)