FastQCFastQC Report
Sun 19 Mar 2023
SRR4895660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483196
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG334326.9189314481080135No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA43050.890942805817929No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26580.5500873351600594No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT13390.27711322113593656No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA8790.18191375756421826No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6830.1413505078684426No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA6390.13224447222245217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT244750.063.005771
AGTGGTA255100.060.2505345
AGCAGTG257400.059.859192
GTGGTAT258100.059.465956
CAGTGGT260300.059.1365934
GGTATCA261000.058.916338
TGGTATC261200.058.7879527
GTATCAA262200.058.6959159
GCAGTGG264400.058.1921463
GTCGTAA450.00964030248.328056145
CATGCGT1201.972312E-742.28705145
GATAGAC1301.6530825E-533.461351
GGTCTGA2705.456968E-1232.218704145
GCCCCAA5350.027.099846145
CGGTCTG1958.7496755E-626.022799145
CGATAGG351.2564937E-424.85700235-39
CGGCTAT651.4879333E-924.537306140-144
AGAGAGT9750.024.535784145
CGACCTA2100.024.16552745-49
CCGGCGT1500.001494107424.16403145
Produced by FastQC (version 0.11.5)