FastQCFastQC Report
Sun 19 Mar 2023
SRR4895659_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895659_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592307
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAA593000.0144.059782
GCTGAAT208150.0143.987523
GAGCTGA593550.0143.92631
GCTGAAG203600.0143.8053
CTGAAGT72850.0143.438114
GCTGAAA173400.0143.356663
CTGAATG77150.0143.108354
CTGAAAT63200.0142.176124
CTGAATT66950.0141.86034
CTGAAGG72850.0141.755254
CTGAAAG66000.0141.716954
CTGAATA64800.0141.670414
CTGAAGA57600.0140.974844
TGAATAG23400.0136.217255
TGAATGA23300.0134.944845
GCTGAAC9900.0133.303513
TGAAGTG25900.0132.53575
TGAAGTA23200.0132.418095
CTGAAAA48200.0132.410354
TGAATGG29750.0132.109865
Produced by FastQC (version 0.11.5)