FastQCFastQC Report
Sun 19 Mar 2023
SRR4895659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592307
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG121512.051469930289529No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28580.4825200445039481No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA17340.2927535889327663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC9480.16005213512587224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8360.14114302211522065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT143750.084.899441
AGTGGTA143600.084.4760065
GGTATCA145200.083.9304668
GTATCAA145850.083.6061259
GTGGTAT145400.083.366276
AGCAGTG147800.082.327712
TGGTATC147800.082.159717
CAGTGGT148350.081.8269654
GCAGTGG151100.080.049783
CGGTCTG2400.042.289772145
CGACCTA2250.025.13220645-49
GCCCCAA7150.024.334574145
TACGCAC2250.023.84337650-54
CCGCAAG6700.023.804903145
TCTCGCA2600.022.30819970-74
CATGGGG33050.021.63057125-29
GAGGTAT2053.665114E-421.2185616
AGGTATC1850.005045800519.5937187
ATGGGGC13850.019.47402425-29
GGTCTGA4102.1633241E-819.450348145
Produced by FastQC (version 0.11.5)