FastQCFastQC Report
Sun 19 Mar 2023
SRR4895658_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895658_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914886
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGT1915750.0144.142681
ACTAGTA1917150.0144.037412
CTAGTAG674550.0143.8473
CTAGTAT655900.0143.572833
CTAGTAA558400.0143.500233
TAGTAGG254700.0135.943954
TAGTAGT254000.0135.836014
TAGTAAG206250.0135.22574
TAGTAGA199300.0135.165624
TAGTATG245750.0134.615974
CTAGTAC35850.0134.153923
TAGTAAT211750.0133.007314
TAGTATA217750.0132.09084
AGTAGTA80100.0130.617575
AGTATTG94000.0130.249635
AGTAGTG86450.0129.614245
AGTATGA73050.0129.601855
AGTAGGT91350.0129.371055
AGTAGAG67750.0128.565465
AGTATAG77550.0128.496985
Produced by FastQC (version 0.11.5)