FastQCFastQC Report
Sun 19 Mar 2023
SRR4895658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914886
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG236131.2331282384434374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA106450.5559077668331169No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT42800.22351200019217854No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG34790.18168183380107222No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA33340.17410958145811292No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC21170.11055488420720608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC20350.10627264495118768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT378850.097.4398651
GGTATCA381100.096.452438
GTGGTAT380900.096.2340856
AGTGGTA381650.096.136025
GTATCAA384950.095.579469
TGGTATC385300.095.194067
AGCAGTG387650.094.984742
CAGTGGT389450.094.2850654
GCAGTGG390050.093.972713
CGGTCTG6900.035.72328145
CGATCCC2008.962161E-932.62376145
GACTAGT5650.030.8009221
CGACCTA5400.025.50842345-49
CGCGAAA5150.023.64958690-94
CATGGGG84500.023.59599525-29
TCGACCG1800.021.75167535-39
ACTAGTA7050.021.5988712
TACGCAC6900.021.01369550-54
CCGCGAA5750.020.9296490-94
TACATGG364350.020.7708420-24
Produced by FastQC (version 0.11.5)