FastQCFastQC Report
Sat 18 Mar 2023
SRR4895657_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895657_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457351
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT24610.5380987469142956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCTG458150.0143.9761
ATCTGAG170800.0143.932283
AATCTGA458400.0143.897482
ATCTGAA124400.0143.590163
ATCTGAT156550.0143.490813
TCTGAAG44700.0143.259874
TCTGATG59300.0143.011754
TCTGAGT62800.0142.735034
CTGAAGT15700.0142.390535
TCTGAGG63000.0142.167434
CTGATAG17050.0142.113075
TCTGAAT44450.0141.632054
CTGAGTG18400.0141.484365
TCTGATT52700.0140.943714
TCTGATA44900.0140.694414
TCTGAGA45550.0140.586534
CTGAATG15500.0140.505835
CTGAGGT22800.0140.432365
CTGAGTA18250.0140.27645
CTGATGT21300.0140.165075
Produced by FastQC (version 0.11.5)