FastQCFastQC Report
Sat 18 Mar 2023
SRR4895657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457351
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG155883.408323147866737No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28400.6209672658417714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA22590.49393135687907097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT13520.29561540261199826No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6810.1489009535345938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC4920.10757601929371532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA161750.073.784445
AAGCAGT163750.073.281781
GTGGTAT163400.072.8982856
GGTATCA164400.072.895868
GTATCAA165100.072.5867849
CAGTGGT165200.072.375224
AGCAGTG165800.072.200772
TGGTATC165250.072.169927
GCAGTGG168850.070.681873
CGGTCTG2150.047.208626145
CTTGCGA657.332909E-444.614746145
CCGCAAG4550.031.867676145
CGATAGG351.2559792E-424.8584235-39
TACGCAC1900.023.65755750-54
GGTCTGA2852.77234E-722.89441145
CATGGGG27050.022.51501825-29
CGACCTA2000.022.47467845-49
TGACGAC1950.022.30737340-44
CGATAGA1900.022.131264105-109
CGCAAGG2653.7729642E-621.886478145
Produced by FastQC (version 0.11.5)