FastQCFastQC Report
Sat 18 Mar 2023
SRR4895656_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895656_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561032
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCAT561450.0144.109541
TGGCATA562000.0143.96852
GGCATAG182400.0143.711183
GGCATAT197700.0143.53213
GGCATAA171300.0143.2993
GCATATG67400.0143.155214
GCATAGA54850.0142.910424
GCATAGT61500.0142.818054
GCATAGG65150.0142.78624
GCATAAG63050.0142.395164
GCATATT68600.0142.122734
CATAGGT22400.0141.97165
GCATAAT59150.0141.664934
GCATATA60900.0140.67284
CATATGT22400.0140.361955
CATAATG21150.0140.133645
GCATAAA50000.0140.042624
CATATGA20850.0139.728915
CATATGG22600.0139.438925
GCATATC2950.0139.336154
Produced by FastQC (version 0.11.5)