FastQCFastQC Report
Sat 18 Mar 2023
SRR4895656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561032
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG196353.4998003678934535No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27650.4928417630366895No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19440.34650429922000886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG8780.1564973120962797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6610.11781859145289396No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG6180.11015414450512627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5980.10658928545965293No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC5960.10623279955510559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT189350.078.005721
AGTGGTA193150.076.351625
GGTATCA194600.075.924978
GTATCAA195050.075.6754769
GTGGTAT195000.075.5461656
AGCAGTG196450.075.1126562
CAGTGGT196900.074.867334
TGGTATC197700.074.6244357
GCAGTGG199800.073.7443853
CGGTCTG2400.060.420013145
CACGGGA2354.2882675E-827.767492145
CGACCTA1400.022.78494345-49
GCCCCAA4505.456968E-1222.556803145
CATGGGG37000.022.45464525-29
CGCGAAA2550.021.03850490-94
CGATAGG1250.020.87929335-39
ATGGGGC13350.020.09295525-29
CCGATAG659.84588E-720.07624440-44
CCTCGTT2800.019.67790435-39
GCTATTC2608.0658174E-519.520311145
Produced by FastQC (version 0.11.5)